Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies.

Insoluble antigen and antibody derivatives were obtained when ethyl chloroformate was added to solutions of antigen or antibody. Evidence has been obtained to show that polymerization has occurred and that the protein molecules are linked covalently to one another. The immunoadsorbent properties of these insolubilized proteins were investigated. They were found to be efficient, specific, and stable immunoadsorbents, and were used to isolate specific antibodies from a number of proteins, to isolate antigens from mixtures, and to remove contaminating antigen and antibody proteins from solutions.